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. 2014 Feb 13;5(2):e1061. doi: 10.1038/cddis.2014.29

Figure 5.

Figure 5

HAGE counteracts the anti-proliferative effect of IFNα in ABCB5+ MMICs. (a, b and c) IF with antibodies to HAGE (red), ABCB5 (green), SOCS1 (green) and PML (green) on FM82 control, FM82 shRNA1 and 2 melanoma spheres. (d) IF and IB with an antibody to PML (or IgG) on FM82 cell lines and corresponding melanoma spheres stably-expressing PML or the empty vector. (e) FM82 empty vector and stably expressing PML spheres formation in the presence or absence of cDNA transfected HAGE or SOCS1. ANOVA: ***P=<0.0001. Scale bar 100 μm (for spheres) and 20 μm (for cells). (f) IF and IB with an antibody to PML on FM82 shRNA/PMLshRNA CTL and FM82shRNA/PMLshRNA 1 and 2. (g) Spheres formation assay from FM82shRNA/PMLshRNA CTL and FM82shRNA/PML shRNA 1 and 2. ANOVA: ***P=<0.0001