Figure 4.

miRNA let-7e is upregulated during differentiation. (Upper) Schematic overview of treatments. Silencing and mimicking miRNA let-7e protocol used in ASC culture. Untreated ASCs were transfected with 100 nM let-7e or control at day 2 of differentiation, and collected at day 6. ATRA-treated cells were transfected with 100 nM antiLet-7e or control anti-miR (CantimiR) at day 4 and collected at day 6. (Lower) Total RNA was extracted from ASCs with or without ATRA and transfected with let-7e, a silencing oligonucleotide (antiLet-7) or their respective negative controls (C/CantimiR). Samples were assayed for miRNA let-7e expression by qRT-PCR and normalized to SU6; n=4. Data are means±S.D. *P<0.05 versus Cmimc; ⁺P<0.05 versus CantimiR