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. 2014 Feb 6;5(2):e1048. doi: 10.1038/cddis.2014.2

Figure 5.

Figure 5

miRNA let-7e influences the expression of early nephrogenic and epithelial markers. ATRA-treated cells were transfected with 100 nM antiLet-7e or control antimir (CantimiR) at day 4 and collected at day 6. RT-PCR of ASCs transfected with 100 nM let-7e shows increased expression of PAX2, Wnt4 and WT1 compared with control cells (C). miRNA let-7e silencing (+antiLet-7e) reduces the expression of early nephrogenic markers PAX2, Wnt4, WT1, megalin and CK18 compared with control antimiR-transfected cells (+CantimiR); n=4. Data are means±S.D. *P<0.05 versus Cmimc; +P<0.05 versus CantimiR. mRNA expression levels were normalized to GAPDH