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. 2014 Feb 13;5(2):e1056. doi: 10.1038/cddis.2014.33

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Involvement of MAPK in the generation of mitochondrial ROS and mitochondrial dysfunction. Apoptosis of FaDu cells was assessed 24 h after NTP with or without preincubation with NAC (10 mM), SB2023580 (10 μM), or SP 600125 (10 μM). (a) Immunoblotting was performed using antibodies against p-JNK, p-p38, p-ERK, cleaved caspase-3, and PARP. (b) Analysis of apoptosis by FACS with Annexin V-PI. (c) Measurement of ROS generation by flow cytometry. (d) Measurement of mitochondrial superoxide using MitoSOX and MitoTracker. (e) Measurement of MMP using JC-1. *P<0.05, **P<0.01, and ***P<0.001

Involvement of MAPK in the generation of mitochondrial ROS and mitochondrial dysfunction. Apoptosis of FaDu cells was assessed 24 h after NTP with or without preincubation with NAC (10 mM), SB2023580 (10 μM), or SP 600125 (10 μM). (a) Immunoblotting was performed using antibodies against p-JNK, p-p38, p-ERK, cleaved caspase-3, and PARP. (b) Analysis of apoptosis by FACS with Annexin V-PI. (c) Measurement of ROS generation by flow cytometry. (d) Measurement of mitochondrial superoxide using MitoSOX and MitoTracker. (e) Measurement of MMP using JC-1. *P<0.05, **P<0.01, and ***P<0.001