Figure 1.

Assessment of the role of DJ-1 in vitro. Modulation of DJ-1 protein in vitro in HL-1 cells by transfection with WT and MitoDJ-1 and non-functional mutant DJ-1 (L166P and Cys106A). (a) Cell survival in response to simulated IRI, n=5/group. (b) MPTP opening in response to laser-induced oxidative stress, n≥5/group. (c) Representative images of TMRM-loaded mitochondria: (i) Control, (ii) WT-DJ (more elongated mitochondria), (iii) Cys106A and (iv) MitoDJ-1 (more elongated mitochondria). (d) Mitochondrial morphology, n≥5/group. Significance assessed by one-way ANOVA; significance indicated against comparison with control (white bars). *P<0.05, **P<0.01