Figure 2.

Chronic ethanol treatment alters the UPS in the cerebral cortices of WT mice, but causes no changes in the cortices of TLR4-KO mice. (a) The immunoblot analysis and quantification of the β5, β2, β1 and α2 subunits expressions in the brain extracts of the WT and TLR4-KO mice treated or not with ethanol for 4 months. (b) The immunoblot analysis and quantification of the β5i, β2i, β1i, PSME1 and PSME2 subunit expressions in the brain extracts of the WT and TLR4-KO mice treated or not with ethanol for 4 months. (c) The mRNA levels of the β5i, β2i and β1i subunits of the WT and TLR4-KO mice treated or not with ethanol for 4 months. (d) The IFN-γ levels of the WT and TLR4-KO mice treated or not with ethanol for 4 months. (e) Levels of the three proteolytic activities, chymotrypsin-like, trypsin-like and caspase-like in the WT and TLR4-KO mice treated or not with ethanol for 4 months. Data represent mean±S.E.M., n=7 mice/group. *P<0.05, **P<0.01 compared with the ethanol-treated WT or TLR4-KO mice with their respective untreated control groups, according to an unpaired Student's t-test. Blots were stripped, and the total quantity of GAPDH was also assessed. A representative immunoblot of each protein is shown