Figure 5.

Inhibition of Smad7 with the specific Smad7 antisense oligonucleotide (AS) reduces the proliferation of neoplastic cells in human CRC explants. Representative pictures of SMAD7-, PCNA-, p-eIF2α Ser51- and CDC25A-stained sections of freshly obtained CRC explants treated with either Smad7 sense oligonucleotide (S) or AS for 24 h. Isotype control stainings are also indicated. The scale bars are 40 μm. The scale bars in the insets are 10 μm. One of five representative experiments in which similar results were obtained is shown