Figure 4.

NSC697923 induces apoptosis through activation of JNK pathway in p53 mutant NB cells. (a) A panel of six NB cell lines were lysed, subjected to SDS–PAGE and immunoblotted with indicated antibodies. (b) SK-N-AS cells were treated with indicated concentrations of NSC697923 for 4 h, lysed, subjected to SDS–PAGE and immunoblotted with indicated antibodies. (c) SK-N-AS cells were left untreated or treated with NSC697923 alone, specific pathway inhibitor alone (SP600125: JNK inhibitor; PS1145: IKK inhibitor; SB203580: p38 inhibitor; AZD8330: ERK inhibitor), or the combination of the two for 24 h, then cell viability was measured by adding 10 μl of CCK-8 and reading the absorbance at 450 nm. Data are represented as mean±S.D. P-value <0.05 (*) was indicated. (d and e) SK-N-AS cells were treated with NSC697923 alone, SP600125 alone, or the combination of the two for the time courses as indicated. Cells were lysed, subjected to SDS–PAGE, and immunoblotted with indicated antibodies. Beta-actin was detected as a loading control. Data from a, c, and e are representative of three independent experiments. Data from b and d are representative of two independent experiments