Figure 5.

Constitutive expression of c-Jun or ALDH mimics the effect of Dkk-1 in parental MOS-J cells. Plasmid constructs encoding human ALDH1A1 or c-Jun were transfected into parental MOSJ cells and subjected to post-confluent tissue culture. (a) Flow cytometric apoptosis assays demonstrating a reduced proportion of apoptotic cells in cultures that received ALDH1A1 or c-Jun cDNA when compared with controls harboring an equivalent vector control. Data were analyzed by ANOVA and Tukey post test after arcsine transformation and means±S.D. are displayed. **P<0.01, ***P<0.005. (b) Aldefluor assay shows increased ALDH activity of transfectants when compared with the parental cells