Figure 8.

H₂O₂-induced oxidative stress decreases FOXC1 levels. (a) Nuclear extracts were resolved by 10% SDS-PAGE and immunoblotted using antibodies against endogenous FOXC1 and TFIID. (b) Total RNA was extracted and FOXC1 and HPRT1 RNA levels were quantified by qPCR. FOXC1 RNA levels were normalized to HPRT1 RNA levels. The * indicates a P value <0.01 (Student's t-test) relative to ‘no H₂O₂' samples. (c) FOXC1 was able to transactivate the luciferase reporter gene under ‘no H₂O₂' conditions. The transactivation ability of FOXC1 significantly decreased (*) in HeLa cells treated with a ‘low dose' and ‘severe dose' of H₂O₂. (d) Immunoblot analysis showed that exogenous FOXC1 protein levels decreased when cells were treated with H₂O₂. Lysates were resolved by 10% SDS-PAGE and immunoblotted using Xpress and tubulin antibodies. Quantification is based on triplicated experiments