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. 2014 Jan 21;22(3):607–622. doi: 10.1038/mt.2013.265

Figure 3.

Figure 3

Engraftment of transduced cells, immune reconstitution, and systemic detoxification in ADA−/− recipients. (a) Vector copy number analysis of thymus, spleen, and bone marrow in ADA−/− recipients. (Mean ± SD). (b) Percentage of donor cells in thymus, spleen, and bone marrow by quantitative PCR (qPCR). Percentage of DNA with Y chromosome were evaluated in sex-mismatched transplants indicated in 1a (Mean ± SD). (c) ADA activity and (d) SAHH activity in red blood cells, BM cells, thymocytes, and lung tissue of ADA−/− transplants and control mice were measured by enzymatic activity assay as indicated (Mean ± SD). (e) Histopathologic analysis of lung sections from ADA−/− transplanted with ADA−/− Lin- gRVSFada/W or ADA−/− Lin- LV EFS ADA or ADA+/+ WT Lin- cells compared to lung sections from 18-day-old untreated ADA−/− and ADA−/− mice under ERT (ADA−/− ERT) mice. All sections have been stained with hematoxylin and eosin.