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. 2013 May 10;59(4):378–384. doi: 10.1262/jrd.2013-015

Table 6. Survival and maturation of immature porcine oocytes after cryoprotectant treatment or vitrification using a combination of EG and PG as permeable cryoprotectants.

Treatment groups Total no. of oocytes examined No. of live oocytes No. of oocytes matured to MII
(% total) (% live oocytes)
Control 245 236 166
(96.3 ± 1.8)a (69.9 ± 2.9)
Toxicity control 245 238 170
(97.3 ± 0.8)a (70.8 ± 3.8)
Vitrified 397 173 119
(42.6 ± 5.3)b (66.4 ± 4.4)

Five replicates were performed. Percentage data are presented as mean ± SEM values. Toxicity control was carried out by subjecting COCs to CPA treatment without cooling. a,b Percentages with different letters in the same column differ significantly (P<0.05). MII = metaphase II, EG = ethylene glycol, PG = propylene glycol, COCs = cumulus-oocyte complexes, CPA = cryoprotective agent.