Table 6. Survival and maturation of immature porcine oocytes after cryoprotectant treatment or vitrification using a combination of EG and PG as permeable cryoprotectants.
| Treatment groups | Total no. of oocytes examined | No. of live oocytes | No. of oocytes matured to MII |
| (% total) | (% live oocytes) | ||
| Control | 245 | 236 | 166 |
| (96.3 ± 1.8)a | (69.9 ± 2.9) | ||
| Toxicity control | 245 | 238 | 170 |
| (97.3 ± 0.8)a | (70.8 ± 3.8) | ||
| Vitrified | 397 | 173 | 119 |
| (42.6 ± 5.3)b | (66.4 ± 4.4) |
Five replicates were performed. Percentage data are presented as mean ± SEM values. Toxicity control was carried out by subjecting COCs to CPA treatment without cooling. a,b Percentages with different letters in the same column differ significantly (P<0.05). MII = metaphase II, EG = ethylene glycol, PG = propylene glycol, COCs = cumulus-oocyte complexes, CPA = cryoprotective agent.