Figure 3. prox1a transcript level is yap1-dose-dependent.

Taking un-injected control as 1 fold change, reduced prox1a expression level in yap1 morphants could be rescued with yap1 mRNA. Higher doses of yap1 mRNA led to elevated prox1a expression. Mismatch control embryos showed no significant deviation from un-injected controls. Quantitative PCR results from yap1 morphants, rescued morphants and yap1 gain-of-function were calculated with delta CT method for fold change. The significant difference of the fold change, as compared to control with assumed fold change of one, was analyzed with two-tailed t-test.