Table 3. Percentage of functional hair cells in L1 neuromast (%).
48 hpf Embryos | Un-injected control | Mismatch MO | 0.1 pmol yap1 MO | 0.05 pmol yap1 MO | yap1 MO + 75 pg yap1 mRNA | yap1 MO + 150 pg yap1 mRNA | 150 pg yap1 mRNA |
---|---|---|---|---|---|---|---|
1 | 4/6 (66.67%) | 4/6 (66.67%) | 0/4 (0%) | 3/4 (75.00%) | 1/3 (33.33%) | 1/2 (50.00%) | 2/6 (33.33%) |
2 | 2/4 (50.0%) | 2/4 (50.0%) | 1/3 (33.33%) | 3/6 (50.0%) | 0/2 (0%) | 2/4 (50.0%) | 3/5 (60.0%) |
3 | 4/6 (66.67%) | 2/4 (50.0%) | 0/2 (0%) | 2/4 (50.0%) | 1/2 (50.0%) | 2/4 (50.0%) | 2/2 (100.0%) |
4 | 1/2 (50.0%) | 3/3 (100%) | 1/3 (33.33%) | 2/4 (50.0%) | 2/5 (40.0%) | 2/4 (50.0%) | 2/4 (50.0%) |
5 | 3/5 (60.0%) | 2/3 (66.67%) | 0/3 (0%) | 3/3 (100.0%) | 2/3 (66.67%) | 2/3 (66.67%) | 2/3 (66.67%) |
6 | 3/4 (75.00%) | 3/3 (100.0%) | 0/2 (0%) | 0/2 (0%) | 0/2 (0%) | 4/4 (100.0%) | 4/4 (100.0%) |
7 | 2/4 (50.0%) | 3/3 (100.0%) | 0/2 (0%) | 2/4 (50.0%) | 0/1 (0%) | 4/6 (66.67%) | 3/6 (50.0%) |
8 | 3/6 (50.0%) | 2/4 (50.0%) | 1/3 (33.33%) | 2/4 (50.0%) | 0/1 (0%) | 1/2 (50.0%) | 2/4 (50.0%) |
9 | 2/2 100 | 2/4 (50.0%) | 1/3 (33.33%) | 2/4 (50.0%) | 1/3 (33.33%) | 2/2 (100.0%) | 3/4 (75.00%) |
10 | 2/4 (50.0%) | 2/3 (66.67%) | 0/2 (0%) | 2/4 (50.0%) | 0/3 (0%) | 2/3 (66.67%) | 2/4 (50.0%) |
Average % | 61.83 ± 16.2 | 70 ± 21.9 | 13.33 ± 17.2 | 52.5 ± 24.8 | 22.33 ± 25.3 | 65 ± 18.9 | 63.5 ± 22.2 |