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. Author manuscript; available in PMC: 2015 Mar 6.
Published in final edited form as: J Mol Biol. 2013 Nov 16;426(5):1077–1094. doi: 10.1016/j.jmb.2013.11.010

Fig. 3.

Fig. 3

13CO REDOR spectra of samples containing FP-Hairpin with a single directly-bonded 13CO/15N spin pair at (A) L7/F8, (B) F8/L9, (C) L12/G13, (D) F11/L12, and (E) I4/G5. Samples A1, B1, and C1 (black spectra) were prepared with initial protein solubilization in Buffer, whereas samples A2, B2, C2, D, and E (red spectra) were prepared with solubilization in Buffer + D-malt. The solid and dotted lines are respectively the total na + lab (S0r) and the na (S1r) spectra. Inset in each figure is the lab spectrum (ΔSr) with deconvolved β sheet peak in green. Spectral processing included Gaussian line broadening of 150 and 100 Hz respectively for A1 and C2 as well as polynomial baseline correction for all spectra. Each S0r and S1r spectrum is the sum of (A1) 76128, (A2) 9328, (B1) 8928, (B2) 36597, (C1) 19856, (C2) 68308, (D) 20000, and (E) 51904 scans.