Skip to main content
. 2013 Sep 25;6(2):227–239. doi: 10.1159/000354304

Fig. 3.

Fig. 3

The ΔpilA mutant is more susceptible to SP-A-mediated opsonization. a- c RAW 264.7 macrophages were infected with either PAO1 or ΔpilA in the presence or absence of hSP-A. The ratio of ingested bacteria was expressed as fold increase in phagocytosed bacteria due to the effect of hSP-A. a Phagocytosis of PAO1, ΔpilA and genetically complemented ΔpilA-comp in the presence of 25 µg/ml hSP-A. b Phagocytosis of PAO1 and ΔpilA in the presence of different concentrations of hSP-A. c Time-dependent phagocytosis of PAO1 versus ΔpilA in the presence of 25 µg/ml hSP-A. d In vivo phagocytosis of PAO1 versus ΔpilA in SP-A+/+ and SP-A−/− mice (n = 3). All phagocytosis experiments were independently performed in triplicate. The mean ± standard deviation from 1 representative experiment is shown. * p < 0.05 when comparing the number of phagocytosed ΔpilA to PAO1.