Figure 5. PKC is required for TPA to induce the formation of protrusions.

On day 4, cultures of MCF10A acini were fed in the absence (control) or presence of 2 µM bisindolylmaleimide I (Bis). TPA or vehicle was added 30 minutes later. Images were captured by phase contrast microscopy (4X) on day 4 before treatment and again 15.5 hours after addition of TPA. Bar  = 100 µM. Arrows indicate protrusions. Representative images are shown.