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. 2014 Mar 5;9(3):e90722. doi: 10.1371/journal.pone.0090722

Figure 8. Laminin staining is disrupted at the bridges between acini.

Figure 8

On day 4, cultures of MCF10A acini were fed in the A) absence or B) and C) presence of 10 nM TPA. The cultures were fixed 23 hours later and stained with DAPI to detect nuclei (blue) and either phalloidin to detect actin (green, left panels) or an anti-laminin antibody (red, right panels) (40X). Bar  = 50 µM. Inserts in B) show regions that were digitally enlarged to show areas of the bridge (in boxes) that have irregular laminin staining (red, right panel); actin staining for the same region is also shown (green, left panels). Arrows in C) show disrupted laminin staining (red, right panel). Representative images are shown.