Figure 5.

Caspase-3/7 activity and cleaved caspase-3 expression in negative control-lentiviral particle (LV-NC) and LV-Zeta cells after γ-irradiation (IR). (a) Caspase-3/7 activities were measured by luminescence in LV-NC^CD133+ and LV-Zeta^CD133+ cells at 96 h after IR. The data are expressed relative to the luminescence of LV-NC cells and were reproduced in three independent experiments (*P<0.05). (b) Cleaved caspase-3 (green) expression was measured by immunofluorescence staining in LV-NC^CD133+ and LV-Zeta^CD133+ cells at 96 h after IR. The nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI; blue). Original magnification, × 200.