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. 2013 Jul 4;5(8):1165–1179. doi: 10.1002/emmm.201302679

Figure 2.

Figure 2

NPGPx deficiency promotes adipogenesis via ROS-dependent dimerization of protein kinas A regulatory subunits and transcription of C/EBP β
  1. Expression of C/EBP β, C/EBP α and PPAR γ measured by qRT-PCR in NPGPx knockdown (KD) and control (V) 3T3-L1 preadipocytes after adipogenic stimulation (n = 5 per group). *p = 0.03, **p = 0.007, ***p = 0.03, #p = 0.04, ##p = 0.008 by independent Student's t-tests.
  2. Western blots of the regulatory subunits of protein kinase A (PKA RI) (B) (non-reducing gel), the monomeric protein kinase A catalytic subunits (PKA C-α) (reducing gel) (C), and phosphorylated CREB (reducing gel) (D) in NPGPx KD and V 3T3-L1 cells after adipogenic stimulation.
  3. Western blots showing the dimer/monomer of PKA regulatory subunits (non-reducing gel, upper panel) and phosphorylated CREB/CREB (reducing gel, lower panel) in NPGPx KD and V 3T3-L1 preadipocytes 30 min after induction with or without N-acetylcysteine (NAC) 10 mM.
  4. C/EBP β expression measured by qRT-PCR in NPGPx KD and V 3T3-L1 preadipocytes 12 h after induction with or without NAC 10 mM (n = 5 per group). *p = 0.002 and #p = 0.02 by independent Student's t-tests. Open bars (□) denote NPGPx knockdown 3T3-L1 cells while filled bars (▪) denote control cells. All values are presented as means ± S.E.M. [Correction added after publication on 4 July 2013: The phrase “by dependent Student's t-tests” in the legend of Fig. 2F has been corrected to “by independent Student's t-tests”].