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. 2013 Jul 4;5(8):1165–1179. doi: 10.1002/emmm.201302679

Figure 4.

Figure 4

Deficiency of NPGPx enhances adipogenesis in vivo
  1. Body weight of NPGPx knockout (KO) mice and wild-type littermates (WT) fed on standard chow diet (C) or high-fat diet (HFD) (n = 27–30 per group). The HFD was started at the age of 10 weeks. *p = 0.017 by repeated measures ANOVA.
  2. Representative photograph inguinal fat pads (upper panel) and gonadal fat pads (lower panel) of 24-week-old NPGPx KO or NPGPx WT mice fed on HFD.
  3. Fat mass of 24 week-old mice measured by micro-CT (n = 10 per group). *p = 0.009 by independent Student's t-test.
  4. Weight of white fat pads (inguinal fat, gonadal fat and mesenteric fat), brown fat pads (BAT) and the liver (n = 10 per group). *p = 0.03, **p = 0.04, #p = 0.009 and ##p = 0.06 by independent Student's t-tests.
  5. Representative haematoxylin and eosin (H&E) staining of the gonadal fat tissue of WT or KO mice fed on HFD.
  6. Adipocyte size and (F) estimated adipocyte number (G) of the gonadal fat pads of 24 week-old mice fed on HFD (n = 15 per group). *p < 0.0001 by independent Student's t-test.
  7. Relative expression of adipogenesis marker genes (pparg, ap2, cd36/fat and aqp7) measured by qRT-PCR in the gonadal fat pads of 24-week-old mice fed on HFD (n = 5 per group). *p = 0.01, **p = 0.02, #p = 0.01, ##p = 0.004 by paired Student's t-test.
  8. Fasting plasma free fatty acid (I), fasting triglycerides (TG) (J), fasting insulin and glucose levels (K) in 24-week-old mice fed on HFD. *p = 0.02, #p = 0.003 and ##p = 0.03 by independent Student's t-tests. Open circles (○), triangles (△) or open bars (□) denote knockout mice. Filled circles (●), filled triangles (▴) or filled bars (▪) denote wild-type mice. All values are presented as means ± S.E.M.