Figure 3.

CYCLON is a MYC target involved in lymphoma progression in vivo

1. Analysis by RT-qPCR and western blot (upper and lower panels, respectively) of CYCLON expression in control and MYC-knockdown Raji lymphoma B cells, n = 3.
2. CYCLON gene expression values in the MYC-inducible cell line, P493 (Schlosser et al, 2005).
3. Affymetrix-derived MYC gene expression values for DLBCL in GEO GSE10846 (Lenz et al, 2008) and GEO GSE4475 (Hummel et al, 2006); high CYCLON expression: above 3rd quartile; low CYCLON expression: below 1st quartile, p-values from Student t test.
4. Kaplan–Meier cumulative survival curves for xenotransplanted mice bearing tumours derived from Raji shCtrl and Raji shCYCLON cell lines, p-value from log-rank test.
5. Tumour weight (28 days post-injection) for xenotransplanted mice bearing tumours derived from Raji shCtrl and Raji shCYCLON cell lines, n = 11 for each group, p-value from Wilcoxon test (left) and bioluminescence imaging for 4 representative mice (right).