Figure 4.

CYCLON regulates gene signatures associated with B-cell differentiation and proliferation

1. Gene Set Enrichment Analysis (GSEA) enrichment plots obtained from gene expression data from CYCLON knockdown Raji cells (shCYCLON, n = 4) compared to control Raji cells (shCTRL, n = 4) with associated heat-maps (left). GSEA enrichment plots show down-regulation of aggressive multiple myeloma signature (A) and up-regulation of a CD40-dependent B-cell signature (B) in CYCLON knock-down compared to control Raji lymphoma B cells. Genes are ordered on the x axis by their differential expression score (each vertical line representing a gene).
2. Top 50 genes up-regulated (red) or down-regulated (blue) upon CYCLON knockdown (shCYCLON) in Raji lymphoma B cells compared to control (shCtrl). Gene sets are from MSIGDB collection. See the Materials and Methods section and supplementary material for detailed data analysis.
3. Heat-map representation of co-regulated genes upon CYCLON knockdown and JQ1 treatment in Raji cells (data from GSE29449, Mertz et al, 2011).
4. Venn diagram showing the overlap between differentially expressed genes upon CYCLON knockdown and JQ1 treatment in Raji cells (data from GSE29449, Mertz et al, 2011) and list of commonly down- and up-regulated genes. Black and green arrows: genes cited in text. Green arrows refer to genes commonly regulated by CYCLON and JQ1 within the CD40 signature.