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. 2013 Aug 5;5(8):1213–1226. doi: 10.1002/emmm.201201929

Figure 6.

Figure 6

In vivo IgA2 EGFR activity is mediated by macrophages in a syngeneic peritoneal model using Ba/F3-EGFR cells
  1. Effector cells in the peritoneum in the Ba/F3-EGFR i.p. model. Effector cell types were identified by FACS and their relative number was determined compared to known amount of beads.
  2. Expression of FcRs on the different effector cell types in the peritoneal lavage during the Ba/F3-EGFR i.p. model in PBS-treated mice. Expression of mouse FcγRs and human FcαRI on macrophages (F4/80+) and PMNs (Ly6Ghigh) was analysed by FACS.
  3. Depletion of macrophages in the Ba/F3-EGFR i.p. model. Specific cytotoxicity of Ba/F3-EGFR cells in FcαRI Tg mice treated with 50 μg IgA2 EGFR or PBS. Macrophages were depleted prior to the experiment by injection of chlodronate liposomes (***p < 0.001, ANOVA, Bonferroni post test, four to five mice/group).
  4. Depletion of PMNs in the Ba/F3-EGFR i.p. model. Specific cytotoxicity of Ba/F3-EGFR cells in FcαRI Tg mice treated with 50 μg IgA2 EGFR or PBS. Prior to the experiment, mice were injected with 250 μg Gr-1 (Ly6G/C-specific) antibody, or with isotype control, two times intraperitoneally to deplete PMNs (***p < 0.001, ANOVA, Bonferroni post test, 9–10 mice/group).