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. 2013 Jul 8;5(8):1227–1246. doi: 10.1002/emmm.201202258

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© 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of EMBO

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Sublethal hypoxia induced STI-1 expression in primary cortical cultures (PCCs)Source data is available for this figure in the Supporting Information.

4 h-hypoxia in PCCs was sublethal. Hypoxia was induced in a two-gas incubator (Jouan, Winchester, Virginia) equipped with an O₂ probe to regulate N₂ levels. Represented images (top panel) and summarized data (bottom left panel) for the number of viable MAP-2⁺ neurons were shown. Summarized data based on lactate dehydrogenase (LDH) assay was also shown (bottom right panel).

The number of PCCs expressing STI-1 (B) and the total level of STI-1 expression in the cultures (C) were measured by means of immunocytochemistry and western blot, respectively. Hypoxia increased the number of PCCs expressing STI-1 (B) and the total protein level of HIF-1α and STI-1 in the cultures (C). Values are mean ± SEM. (*p < 0.05 and **p < 0.01 versus control). Scale bar, 50 µm.