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. Author manuscript; available in PMC: 2015 Mar 6.
Published in final edited form as: J Mol Biol. 2013 Sep 13;426(5):1061–1076. doi: 10.1016/j.jmb.2013.09.007

Fig. 3. Enrichment of the B1 and B2.3/4 virions from wheat or barley and characterization of their physiochemical properties.

Fig. 3

A) Northern blot demonstrating the enrichment of the subsets of the three BMV virions. The proportion of each RNA in the purified virions is below the blot image. B) Isoelectric focusing chromatographs of the subsets of the BMV virions isolated from barley and wheat. The peaks near pI of 5.0 and 8.0 are pI standards added to each sample. C) The difference (ΔTmApp) in apparent melting temperature of the capsid for the subsets of the BMV virions isolated from barley and wheat as a function of pH. The upper panel compares subsets of BMV enriched for the same RNAs but isolated from different hosts. The lower panel compares the subsets of BMV isolated from the same host but enriched for different RNAs.