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. 2014 Feb 18;4(2):e363. doi: 10.1038/tp.2013.132

Figure 5.

Figure 5

Maternal immune activation (MIA) reduces survival of new born cells and affects differentiation morphology of neuronal cells in the dentate gyrus (DG) of the hippocampus. Total number of 5-bromodeoxyuridine (BrdU)-labeled cells in the DG. (a) Quantification of surviving BrdU-positive (BrdU+) cells show significantly lower number of surviving cells in PIC mice. Sample images (20 × ) depict a significant difference in the number of surviving BrdU-labeled cells between (b) control and (c) PIC animals. (d) Schematic and sample pictures (63 × ) illustrating differentiation analysis by morphological criteria using doublecortin (DCX) staining. (e) Percentages of BrdU+ cells in different stages of developmental morphology during the DCX period. (f) PIC animals show significantly lower number of BrdU+/NeuN+ cells. (g) No differences observed in BrdU+/glial fibrillary acidic protein (GFAP)+ cells. n=5-6 animals per group. Gray scale bars denote 50 μm, yellow scale bars denote 5 μm. Data represent mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, n.s. not significant.