A, Quantitative RT-PCR analysis of HES1 and HEY1 expression in the original study cohort of 44 HNSCC tumors and 25 normal mucosa. Expression of HES1 and HEY1 were significantly higher in HNSCC tumor tissues in comparison to normal tissues. Experiments were performed by Taqman realtime RT-PCR in triplicate and the data were normalized to ACTIN per sample. B, C and D, Quantitative RT-PCR analysis of HES1 and HEY1 (B), and JAG1 (C) expression in a separate cohort of 31 HNSCC tumors and 17 normal mucosa, and JAG2 expression in a additional cohort of 63 HNSCC tumors and 30 normal mucosa (D). Expression of HES1, HEY1, JAG1, and JAG2 were significantly higher in HNSCC tumor tissues in comparison to normal tissues. Experiments were performed by Taqman realtime RT-PCR in triplicate and the data were normalized to ACTIN or GAPDH per sample.