Figure 8.

Effects of asymmetric dimethylarginine (ADMA) infusion and dimethylarginine dimethylaminohydrolase (DDAH)-1 overexpression on ischemia/reperfusion (IR)-induced acute tubular necrosis (ATN). (a) The upper panel shows the representative renal histology with periodic acid–Schiff (PAS) staining. (b) The lower panel shows the quantitative data. Magnification × 200. The renal histology stained with PAS reagent. Renal damage included detachment of epithelial cells of the tubuli, interstitial edema, and many tubular cell casts. A higher score, therefore, reflects a higher degree of renal injury. IR-injured kidneys increased the intensity of renal damage. ADMA infusion further augmented the intensity, which was prevented in DDAH-1 transgenic (DDAH-1 Tg) mice.