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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Microb Pathog. 2013 Dec 17;66:36–39. doi: 10.1016/j.micpath.2013.12.002

Figure 2.

Figure 2

The effects of L-lactate on CT production. Overnight cultures of V. cholerae O395N1 and its respective isogenic ΔnqrA-F mutant were washed and diluted in LB (initial pH 6.5) to OD 600 = 0.05 and shaken in LB (initial pH 6.5) at 30°C for overnight. The cell-free culture supernatants were prepared after growth and assayed for CT production by CT-ELISA. All experiments were repeated at least three times. The error bars indicate standard errors. P values were calculated by one-way ANOVA followed by post hoc comparisons using the Bonferroni test and * indicates statistical significance (P< 0.05).