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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Microb Pathog. 2013 Dec 17;66:36–39. doi: 10.1016/j.micpath.2013.12.002

Figure 3.

Figure 3

The effects of HQNO and TTFA on CT production. Overnight cultures of V. cholerae O395N1 and CA401 were washed and diluted in LB (initial pH 6.5) to the initial OD 600 = 0.05 and cultured in LB (initial pH 6.5) at 30°C for 8hr. Cultures of V. cholerae N16961 were grown in YEP medium under AKI conditions. HQNO and TTFA were added at 2.5 μM. The cell-free culture supernatants were prepared after growth and assayed for CT production by CT-ELISA. All experiments were repeated three times. The error bars indicate standard errors. P values were calculated by one-way ANOVA followed by post hoc Bonferroni test and * indicates P< 0.05.