Source organism |
Pseudomonas sp. strain ADP |
DNA source |
GenScript, based on accession No. U66917
|
Gene sequence encoding His-tagged full-length AtzF |
atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgaatgaccgcgcgccccaccctgaaagatctggtcgagtcacgccggatcacctgaccgatctggcttcctatcaggctgcctatgccgccggtacagacgccgccgacgtcatttcggacctgtatgcccgtatcaaagaagacggcgaaaatccgatctggattagcctgttgcccttggaaagcgcattggcgatgctggccgacgcgcagcaacgcaaggacaagggagaagcgttgccgctctttggcatccccttcggcgtcaaggacaacatcgacgtcgcaggccttccgacgactgccgggtgtacggggttcgcgcgtacgccccgacagcacgccttcgtcgtacagcgcctggtggacgctggcgcgatcccgatcggaaaaacgaacctcgatcaattcgcgaccgggttgaacggcactcgcacgccgtttggcattccgcgctgcgtgttcaacgagaactacgtatccggcggctccagcagtggctccgcagtggccgtcgccaacggcacggtaccgttctcgctcgggacggacactgccggttccggccgcattcctgctgcgttcaacaatctggtgggcttgaaaccgaccaaaggcctgttctcgggcagtggactggttcccgcggcgcgaagccttgactgcatcagcgtcctcgcccataccgtagatgacgcccttgcggtcgcacgcgtcgccgccggctacgatgctgatgacgctttttcgcgcaaggcgggcgccgccgcactgacagaaaagagttggcctcgtcgcttcaatttcggggtcccagcggcggaacatcgccagtttttcggtgacgcggaagccgaggcgcttttcaataaagcggttcgcaagcttgaagagatgggtggcacctgcatctcgtttgactatacccccttcaggcaggctgctgaactgctctacgccggcccttgggttgcggagcgcctggcggccatcgagagccttgcggacgagcatcccgaggtgctccacccggtcgttcgtgacatcatcttgtccgcgaagcgaatgagcgcagtcgacacgttcaacggtatctatcgcctggccgaccttgtcagggctgcagagagcacttgggaaaagatcgatgtgatgctgctgccgacggcgccgaccatctacactgtagaagacatgctcgccgatccggtacgcctcaacagcaatctgggcttctacacgaacttcgtgaacttgatggatttgtccgcgattgctgttcccgcaggcttccgaaccaatggcctgccatttggcgtcactttcatcggtcgggcgttcgaagatggggcgatcgcaagcttgggaaaagctttcgtggagcacgacctcgccaagggcaacgcggccacggcggcgccacccaaggataccgtcgcaatcgccgtggtaggtgcacatctctccgaccagcccttgaatcatcagctcacggagagcggcggaaagctacgggcaacaacgcgtactgcgccgggatatgccttgtacgcactccgtgatgcgacgccggctaagcctggaatgttgcgcgaccagaatgcggtcgggagcatcgaagtggaaatctgggatctgccggtcgccgggttcggtgcgtttgtaagtgaaattccggcgccgttgggtatcgggacaataacactcgaagacggcagccatgtgaaaggctttctgtgcgagccacatgccatcgagacggcgctcgacatcactcactacggcggctggcgagcatacctcgcggctcaatag |
Gene sequence encoding His-tagged AtzF467
|
atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgaatgaccgcgcgccccaccctgaaagatctggtcgagtcacgccggatcacctgaccgatctggcttcctatcaggctgcctatgccgccggtacagacgccgccgacgtcatttcggacctgtatgcccgtatcaaagaagacggcgaaaatccgatctggattagcctgttgcccttggaaagcgcattggcgatgctggccgacgcgcagcaacgcaaggacaagggagaagcgttgccgctctttggcatccccttcggcgtcaaggacaacatcgacgtcgcaggccttccgacgactgccgggtgtacggggttcgcgcgtacgccccgacagcacgccttcgtcgtacagcgcctggtggacgctggcgcgatcccgatcggaaaaacgaacctcgatcaattcgcgaccgggttgaacggcactcgcacgccgtttggcattccgcgctgcgtgttcaacgagaactacgtatccggcggctccagcagtggctccgcagtggccgtcgccaacggcacggtaccgttctcgctcgggacggacactgccggttccggccgcattcctgctgcgttcaacaatctggtgggcttgaaaccgaccaaaggcctgttctcgggcagtggactggttcccgcggcgcgaagccttgactgcatcagcgtcctcgcccataccgtagatgacgcccttgcggtcgcacgcgtcgccgccggctacgatgctgatgacgctttttcgcgcaaggcgggcgccgccgcactgacagaaaagagttggcctcgtcgcttcaatttcggggtcccagcggcggaacatcgccagtttttcggtgacgcggaagccgaggcgcttttcaataaagcggttcgcaagcttgaagagatgggtggcacctgcatctcgtttgactatacccccttcaggcaggctgctgaactgctctacgccggcccttgggttgcggagcgcctggcggccatcgagagccttgcggacgagcatcccgaggtgctccacccggtcgttcgtgacatcatcttgtccgcgaagcgaatgagcgcagtcgacacgttcaacggtatctatcgcctggccgaccttgtcagggctgcagagagcacttgggaaaagatcgatgtgatgctgctgccgacggcgccgaccatctacactgtagaagacatgctcgccgatccggtacgcctcaacagcaatctgggcttctacacgaacttcgtgaacttgatggatttgtccgcgattgctgttcccgcaggcttccgaaccaatggcctgccatttggcgtcactttcatcggtcgggcgttcgaagatggggcgatcgcaagcttgggaaaagctttcgtggagcacgacctcgccaag |
Forward primer (AtzF Fwd A) |
ATCATCACAGCAGCGGCCTG |
Reverse primer (AtzF Rev Trunc) |
GTTTGGTTGGATCCTCATTACTTGGCGAGGTCGTGCTCCACGAAAGC |
Cloning vector |
pUC57 |
Expression vector |
pETCC2, a pET-14b derivative (Peat et al., 2013 ▶) |
Expression host |
E. coli BL21 (λDE3), Invitrogen |
Complete amino-acid sequence of AtzF |
MGSSHHHHHHSSGLVPRGSHmndraphpersgrvtpdhltdlasyqaayaagtdaadvisdlyarikedgenpiwisllplesalamladaqqrkdkgealplfgipfgvkdnidvaglpttagctgfartprqhafvvqrlvdagaipigktnldqfatglngtrtpfgiprcvfnenyvsggsssgsavavangtvpfslgtdtagsgripaafnnlvglkptkglfsgsglvpaarsldcisvlahtvddalavarvaagydaddafsrkagaaaltekswprrfnfgvpaaehrqffgdaeaealfnkavrkleemggtcisfdytpfrqaaellyagpwvaerlaaiesladehpevlhpvvrdiilsakrmsavdtfngiyrladlvraaestwekidvmllptaptiytvedmladpvrlnsnlgfytnfvnlmdlsaiavpagfrtnglpfgvtfigrafedgaiaslgkafvehdlakgnaataappkdtvaiavvgahlsdqplnhqltesggklrattrtapgyalyalrdatpakpgmlrdqnavgsieveiwdlpvagfgafvsepaplgigtitledgshvkgflcephaietaldithyggwraylaaq |
Complete amino-acid sequence of AtzF467
|
MGSSHHHHHHSSGLVPRGSHmndraphpersgrvtpdhltdlasyqaayaagtdaadvisdlyarikedgenpiwisllplesalamladaqqrkdkgealplfgipfgvkdnidvaglpttagctgfartprqhafvvqrlvdagaipigktnldqfatglngtrtpfgiprcvfnenyvsggsssgsavavangtvpfslgtdtagsgripaafnnlvglkptkglfsgsglvpaarsldcisvlahtvddalavarvaagydaddafsrkagaaaltekswprrfnfgvpaaehrqffgdaeaealfnkavrkleemggtcisfdytpfrqaaellyagpwvaerlaaiesladehpevlhpvvrdiilsakrmsavdtfngiyrladlvraaestwekidvmllptaptiytvedmladpvrlnsnlgfytnfvnlmdlsaiavpagfrtnglpfgvtfigrafedgaiaslgkafvehdlak |