Table 1. DNA and protein used in this study.
The nucleotide and peptide sequences for the artificial His tag used for the purification of AtzF and AtzF467 are shown in bold.
| Source organism | Pseudomonas sp. strain ADP |
| DNA source | GenScript, based on accession No. U66917 |
| Gene sequence encoding His-tagged full-length AtzF | atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgaatgaccgcgcgccccaccctgaaagatctggtcgagtcacgccggatcacctgaccgatctggcttcctatcaggctgcctatgccgccggtacagacgccgccgacgtcatttcggacctgtatgcccgtatcaaagaagacggcgaaaatccgatctggattagcctgttgcccttggaaagcgcattggcgatgctggccgacgcgcagcaacgcaaggacaagggagaagcgttgccgctctttggcatccccttcggcgtcaaggacaacatcgacgtcgcaggccttccgacgactgccgggtgtacggggttcgcgcgtacgccccgacagcacgccttcgtcgtacagcgcctggtggacgctggcgcgatcccgatcggaaaaacgaacctcgatcaattcgcgaccgggttgaacggcactcgcacgccgtttggcattccgcgctgcgtgttcaacgagaactacgtatccggcggctccagcagtggctccgcagtggccgtcgccaacggcacggtaccgttctcgctcgggacggacactgccggttccggccgcattcctgctgcgttcaacaatctggtgggcttgaaaccgaccaaaggcctgttctcgggcagtggactggttcccgcggcgcgaagccttgactgcatcagcgtcctcgcccataccgtagatgacgcccttgcggtcgcacgcgtcgccgccggctacgatgctgatgacgctttttcgcgcaaggcgggcgccgccgcactgacagaaaagagttggcctcgtcgcttcaatttcggggtcccagcggcggaacatcgccagtttttcggtgacgcggaagccgaggcgcttttcaataaagcggttcgcaagcttgaagagatgggtggcacctgcatctcgtttgactatacccccttcaggcaggctgctgaactgctctacgccggcccttgggttgcggagcgcctggcggccatcgagagccttgcggacgagcatcccgaggtgctccacccggtcgttcgtgacatcatcttgtccgcgaagcgaatgagcgcagtcgacacgttcaacggtatctatcgcctggccgaccttgtcagggctgcagagagcacttgggaaaagatcgatgtgatgctgctgccgacggcgccgaccatctacactgtagaagacatgctcgccgatccggtacgcctcaacagcaatctgggcttctacacgaacttcgtgaacttgatggatttgtccgcgattgctgttcccgcaggcttccgaaccaatggcctgccatttggcgtcactttcatcggtcgggcgttcgaagatggggcgatcgcaagcttgggaaaagctttcgtggagcacgacctcgccaagggcaacgcggccacggcggcgccacccaaggataccgtcgcaatcgccgtggtaggtgcacatctctccgaccagcccttgaatcatcagctcacggagagcggcggaaagctacgggcaacaacgcgtactgcgccgggatatgccttgtacgcactccgtgatgcgacgccggctaagcctggaatgttgcgcgaccagaatgcggtcgggagcatcgaagtggaaatctgggatctgccggtcgccgggttcggtgcgtttgtaagtgaaattccggcgccgttgggtatcgggacaataacactcgaagacggcagccatgtgaaaggctttctgtgcgagccacatgccatcgagacggcgctcgacatcactcactacggcggctggcgagcatacctcgcggctcaatag |
| Gene sequence encoding His-tagged AtzF467 | atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgaatgaccgcgcgccccaccctgaaagatctggtcgagtcacgccggatcacctgaccgatctggcttcctatcaggctgcctatgccgccggtacagacgccgccgacgtcatttcggacctgtatgcccgtatcaaagaagacggcgaaaatccgatctggattagcctgttgcccttggaaagcgcattggcgatgctggccgacgcgcagcaacgcaaggacaagggagaagcgttgccgctctttggcatccccttcggcgtcaaggacaacatcgacgtcgcaggccttccgacgactgccgggtgtacggggttcgcgcgtacgccccgacagcacgccttcgtcgtacagcgcctggtggacgctggcgcgatcccgatcggaaaaacgaacctcgatcaattcgcgaccgggttgaacggcactcgcacgccgtttggcattccgcgctgcgtgttcaacgagaactacgtatccggcggctccagcagtggctccgcagtggccgtcgccaacggcacggtaccgttctcgctcgggacggacactgccggttccggccgcattcctgctgcgttcaacaatctggtgggcttgaaaccgaccaaaggcctgttctcgggcagtggactggttcccgcggcgcgaagccttgactgcatcagcgtcctcgcccataccgtagatgacgcccttgcggtcgcacgcgtcgccgccggctacgatgctgatgacgctttttcgcgcaaggcgggcgccgccgcactgacagaaaagagttggcctcgtcgcttcaatttcggggtcccagcggcggaacatcgccagtttttcggtgacgcggaagccgaggcgcttttcaataaagcggttcgcaagcttgaagagatgggtggcacctgcatctcgtttgactatacccccttcaggcaggctgctgaactgctctacgccggcccttgggttgcggagcgcctggcggccatcgagagccttgcggacgagcatcccgaggtgctccacccggtcgttcgtgacatcatcttgtccgcgaagcgaatgagcgcagtcgacacgttcaacggtatctatcgcctggccgaccttgtcagggctgcagagagcacttgggaaaagatcgatgtgatgctgctgccgacggcgccgaccatctacactgtagaagacatgctcgccgatccggtacgcctcaacagcaatctgggcttctacacgaacttcgtgaacttgatggatttgtccgcgattgctgttcccgcaggcttccgaaccaatggcctgccatttggcgtcactttcatcggtcgggcgttcgaagatggggcgatcgcaagcttgggaaaagctttcgtggagcacgacctcgccaag |
| Forward primer (AtzF Fwd A) | ATCATCACAGCAGCGGCCTG |
| Reverse primer (AtzF Rev Trunc) | GTTTGGTTGGATCCTCATTACTTGGCGAGGTCGTGCTCCACGAAAGC |
| Cloning vector | pUC57 |
| Expression vector | pETCC2, a pET-14b derivative (Peat et al., 2013 ▶) |
| Expression host | E. coli BL21 (λDE3), Invitrogen |
| Complete amino-acid sequence of AtzF | MGSSHHHHHHSSGLVPRGSHmndraphpersgrvtpdhltdlasyqaayaagtdaadvisdlyarikedgenpiwisllplesalamladaqqrkdkgealplfgipfgvkdnidvaglpttagctgfartprqhafvvqrlvdagaipigktnldqfatglngtrtpfgiprcvfnenyvsggsssgsavavangtvpfslgtdtagsgripaafnnlvglkptkglfsgsglvpaarsldcisvlahtvddalavarvaagydaddafsrkagaaaltekswprrfnfgvpaaehrqffgdaeaealfnkavrkleemggtcisfdytpfrqaaellyagpwvaerlaaiesladehpevlhpvvrdiilsakrmsavdtfngiyrladlvraaestwekidvmllptaptiytvedmladpvrlnsnlgfytnfvnlmdlsaiavpagfrtnglpfgvtfigrafedgaiaslgkafvehdlakgnaataappkdtvaiavvgahlsdqplnhqltesggklrattrtapgyalyalrdatpakpgmlrdqnavgsieveiwdlpvagfgafvsepaplgigtitledgshvkgflcephaietaldithyggwraylaaq |
| Complete amino-acid sequence of AtzF467 | MGSSHHHHHHSSGLVPRGSHmndraphpersgrvtpdhltdlasyqaayaagtdaadvisdlyarikedgenpiwisllplesalamladaqqrkdkgealplfgipfgvkdnidvaglpttagctgfartprqhafvvqrlvdagaipigktnldqfatglngtrtpfgiprcvfnenyvsggsssgsavavangtvpfslgtdtagsgripaafnnlvglkptkglfsgsglvpaarsldcisvlahtvddalavarvaagydaddafsrkagaaaltekswprrfnfgvpaaehrqffgdaeaealfnkavrkleemggtcisfdytpfrqaaellyagpwvaerlaaiesladehpevlhpvvrdiilsakrmsavdtfngiyrladlvraaestwekidvmllptaptiytvedmladpvrlnsnlgfytnfvnlmdlsaiavpagfrtnglpfgvtfigrafedgaiaslgkafvehdlak |