. 2014 Mar 6;4:4292. doi: 10.1038/srep04292

Table 3. Substrate specificities of family S46 peptidases.

Enzyme	Hydrolysed substrate	Un- hydrolysed substrate
DAP BII¹³	Asp-Arg -\|- Val-Tyr -\|- Ile-His -\|- Pro-Phe -\|- His-Leu
	Lys-Ile -\|- Pro-Tyr -\|- Ile-Leu
	Phe-Leu -\|- Pro-Leu -\|- Ile-Leu-Gly-Lys-Leu-Val-Lys-Gly-Leu-Leu
	Ala-Ala -\|- pNA, Lys-Ala -\|- MCA	Gly-Arg-pNA, Arg-Arg-MNA
	Gly-Phe -\|- pNA	Gly-Pro-MNA
	Ser-Tyr -\|- βNA
PgDPP7²¹^,²³^,²⁴	Trp-Ala -\|- Gly-Gly-Asp-Ala-Ser-Gly-Glu	Trp-His-Trp-Leu-Glu-Leu-Lys-Pro-Gly-Glu-Pro-Met-Tyr
	Ile-Ala -\|- Arg-Arg-His-Pro-Tyr-Phe-Leu	Ser-Pro-Tyr-Ser-Ser-Glu-Thr-Thr
	Lys-Ile -\|- Ala-Gly-Tyr-His-Leu-Glu-Leu	Ala-Pro-Val-Arg-Ser-Leu
	Phe-Leu -\|- Arg-Glu-Pro-Val-Ile-Phe-Leu	Gln-Lys-Gln-Met-Ser-Asp-Arg-Arg-Glu
	Ala-Ala -\|- pNA, Lys-Ala -\|- MCA	Ala-Gly-pNA, Gly-Gly-pNA, Gly-Gly-MCA
	Ala-Phe -\|- pNA, Gly-Phe -\|- pNA	His-Asp-MCA
	Met-Leu -\|- MCA	Gly-Glu-pNA
	Leu-Asp -\|- MCA, Val-Asp -\|- MCA	Gly-Arg-pNA
	Thr-Asp -\|- MCA, Ile-Asp -\|- MCA	Gly-Lys-pNA
	Leu-Glu -\|- MCA	Lys-Lys-MCA
	Leu-Arg -\|- MCA, Val-Arg -\|- MCA	Gly-Pro-MCA
	Arg-Arg -\|- MCA, Gly-Arg -\|- MCA	Ala-Pro-pNA
	Leu-Lys -\|- MCA
	Leu-Gln -\|- MCA, Ala-Asn -\|- MCA
	Thr-Ser -\|- MCA, Ser-Tyr -\|- MCA
PgDPP11²²^,²³	Leu-Asp -\|- MCA, Ile-Asp -\|- MCA	ac-Leu-Asp-MCA
	Leu-Glu -\|- MCA	Lys-Ala-MCA
		Gly-Gly-MCA
		Met-Leu-MCA
		Leu-Arg-MCA, Val-Arg-MCA, Gly-Arg-MCA
		Leu-Lys-MCA, Lys-Lys-MCA
		Gly-Pro-MCA
SpDPP11²³	Lys-Ala -\|- MCA	Gly-Gly-MCA
	Lys-Asp -\|- MCA	Met-Leu-MCA
	Lys-Glu -\|- MCA	Gly-Arg-MCA, Leu-Arg-MCA, Val-Arg-MCA
	Gly-Pro -\|- MCA	Leu-Lys-MCA, Lys-Lys-MCA

Hydrolytic cleavage bond, -|-; uncleaved bond, -; MCA, 7-methoxycoumarin; MNA, 4-methoxy-2-naphthylamide; βNA, β-naphthylamide.