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. 2014 Mar 5;9(3):e90698. doi: 10.1371/journal.pone.0090698

Figure 7. Activation of the BMRF2 promoter by Rta via the AP-1 binding site.

Figure 7

(A) The BMRF2 reporter plasmid and a mutant version. 293T cells were cotransfected with pCMV-R and a reporter plasmid, pBMRF2-AP1, pBMRF2-mAP1, or pGL2-Basic. Luciferase activities were examined at 24 h after transfection. (B) P3HR1 cells that had been treated with TPA and sodium butyrate for 48 h (lytic) or DMSO (latent) were analyzed by ChIP assay using anti-Rta, anti-MCAF1 and anti-ATF2 antibodies. Anti-IgG antibody was used as a control. The binding of ATF2, MCAF1 and Rta to the AP-1 sequence in the BMRF2 promoter was examined by qPCR. Error bars represent standard error. The p values from each experiment were derived using the Student's t-test. * p<0.05, ** p<0.001. Luc: luciferase gene.