Table 2.
Functional categories and genes regulated in Atlantic salmon reared at high temperature for 21 and 56 days identified from microarray analysis
| Probe number | Name (best blast hit) | Day 21 | Day 56 |
|---|---|---|---|
|
Unfolded protein response | |||
|
CA356940 |
Heat shock protein 47 kDa |
1,15 |
1,35 |
|
CA373890 |
Heat shock protein HSP 90-beta-1 |
0,66 |
0,67 |
| EST1-3A_F08 |
Heat shock protein HSP 90-beta-2 |
0,83 |
0,80 |
| EXOB1_E03 |
Eukaryotic translation initiation factor 3 subunit 5 |
0,55 |
0,30 |
| EXOB1_E08 |
Eukaryotic translation initiation factor 3 subunit 6-1 |
0,44 |
0,35 |
|
CA382570 |
Mitogen-activated protein kinase 13 |
0,29 |
0,57 |
| EST1-3A_H06 |
Transcription factor jun-B-1 |
0,43 |
1,02 |
|
CA368716 |
Membrane-bound transcription factor site 2 protease |
0,58 |
0,36 |
|
CA378435 |
Protein phosphatase 2C delta isoform |
0,62 |
0,23 |
|
Tissue remodeling and cytoskeleton | |||
| est03c04 |
Matrix metalloproteinase-9 |
0,34 |
0,51 |
| EXOB3_H01 |
Matrix metalloproteinase-13 |
0,47 |
0,81 |
|
CA378743 |
Fibronectin precursor |
0,44 |
0,29 |
| utu04c11 |
Collagen alpha 1(I) chain-2 |
-0,32 |
-0,95 |
| HK0003_C02 |
Collagen alpha 1(I) chain-1 |
-0,16 |
-0,51 |
| utu02b11 |
Collagen a3(I)-2 |
-0,29 |
-0,56 |
| utu02a06 |
Collagen a3(I)-1 |
-0,22 |
-0,41 |
| HKT0001_B03 |
Alpha 2 type I collagen-1 |
-0,54 |
-0,91 |
| HK0003_E07 |
Myosin light chain 2-1 |
-0,50 |
-0,73 |
| utu02c02 |
Myosin heavy chain 1-1 |
-0,51 |
-0,54 |
| utu04f06 |
Myosin heavy chain 1-2 |
-0,64 |
-0,95 |
| HK0002_F05 |
Myosin heavy chain, fetal |
-0,56 |
-0,50 |
| HK0002_B06 |
Troponin T-3 |
-0,81 |
-0,67 |
| HK0003_D01 |
Troponin C-1 |
-0,71 |
-0,17 |
| HKT0001_E07 |
Actin, alpha 1 |
-0.39 |
-0.71 |
| utu04d04 |
Actin, alpha 4 |
-0.13 |
-0.67 |
| utu04f08 |
Actin, alpha 5 |
-0.58 |
-1.27 |
| HK0003_C08 |
Parvalbumin alpha-2 |
-0,78 |
-0,65 |
| est01e10 |
Tolloid-like protein (nephrosin)-1 |
-0,74 |
-0,47 |
|
Energy metabolism | |||
| HKT0001_H05 |
Cytochrome b-3 |
0,32 |
0,41 |
| utu02b07 |
Cytochrome c oxidase subunit II |
0,59 |
0,44 |
| HK0001_G02 |
ATP synthase beta chain-2 |
-0,43 |
-0,71 |
| HK0002_G02 |
Creatine kinase, sarcomeric mitochondrial precursor |
-0,41 |
-0,63 |
| est03a08 |
Cytochrome c-1 |
-0,66 |
-0,99 |
| HK0003_B03 |
Cytochrome c-2 |
-0,91 |
-1,16 |
| EXOB1_C10 |
Cytochrome P450 2 K4-2 |
-0,94 |
-1,39 |
| HK0002_A12 |
NADH-ubiquinone oxidoreductase 15 kDa subunit |
-0,45 |
-0,43 |
|
Heme biosynthesis | |||
| EXOB2_B10 |
Hemoglobin beta chain Omy 30073 |
-0,54 |
-1,64 |
| HST0001_C04 |
Hemoglobin alpha chain Omy 11839 |
-0,96 |
-1,56 |
| EXOB4_H06 |
Alpha-globin 1-3 Omy 8146 |
-0,76 |
-1,41 |
| HST0001_C02 |
Alpha-globin I-1 Omy 11839 |
-0,18 |
-0,97 |
| utu01e09 |
Embryonic alpha-type globin2 + collagen alpha 2(1) |
-0,43 |
-0,68 |
| HST0001_D08 |
Beta-globin Omy 9744 |
-0,79 |
-1,06 |
| est01g04 |
5-aminolevulinate synthase |
0,08 |
-0,52 |
|
CA381045 |
Aminolevulinate, delta-, synthase 1 |
-0,84 |
-1,23 |
| HK0001_D09 |
Cytochrome P450 2 F1 |
-0,87 |
-1,15 |
|
Immune response | |||
| EXOB1_F11 |
CD9 |
0,45 |
0,36 |
|
CA388403 |
CD9-like |
0,20 |
0,54 |
|
CA378736 |
Tyrosine-protein kinase BTK |
0,44 |
0,29 |
|
CA382425 |
B-cell translocation gene 1-2 |
0,49 |
0,42 |
| EXOB4_C11 |
High affinity immunoglobulin γ Fc receptor I precursor |
0,47 |
0,39 |
|
CA362806 |
Gamma-interferon inducible lysosomal thiol reductase |
0,51 |
0,17 |
|
CA355488 |
Tapasin-2 |
0,40 |
0,45 |
|
CA373659 |
Mannan-binding lectin serine protease 2-2 |
0,25 |
0,46 |
| ENH2_B05 |
Acute phase protein |
0,41 |
0,53 |
|
CA370329 |
Lysozyme C precursor |
0,87 |
0,61 |
|
CA362419 |
Complement component C6 |
0,47 |
0,40 |
| HK0001_F01 |
Complement factor H-1 |
-0,82 |
-0,73 |
|
CA370696 |
Complement control protein factor I-B |
-0,71 |
0,23 |
| EXOB1_E12 |
Serine protease-like protein-3 |
-0,34 |
-0,53 |
| EST1-3A_A09 |
Serine protease-like protein-2 |
-0,42 |
-0,65 |
| EXOB3_B01 |
Cathepsin C-3 |
-0,90 |
-1,30 |
|
CA377504 |
Cold autoinflammatory syndrome 1 protein |
-0,81 |
-0,62 |
| HK0002_G10 |
T-cell receptor α chain V region HPB-MLT precursor (Fr) |
-0,91 |
-0,52 |
|
CA372428 |
Leukotriene B4 receptor 1 |
-1,11 |
-0,82 |
| HK0002_G11 |
Myristoylated alanine-rich protein kinase C substrate |
-1,07 |
-0,87 |
| EXOB2_G01 |
Leukocyte cell-derived chemotaxin 2 |
-0,97 |
-0,86 |
|
CA343700 |
CXC chemokine receptor transcript variant B |
-0,52 |
-0,88 |
| CA361151 | Annexin A1-2 | -0,58 | -0,47 |
Expression values are log2-expression ratios (19°C versus 14°C) from analysis of pooled heart samples (per temperature treatment: 3 fish per triplicate tanks, n = 9).