FIG 2 .

Contribution of RicR-regulated genes to virulence in mice. (A) CFU from lungs and spleens harvested on days 1 (n = 3) and 21 (n = 4) and at ~8 weeks (n = 4) from WT C57BL/6 mice infected with M. tuberculosis H37Rv RicR regulon single mutants. The initial dose of infection was ~500 to 1,000 CFU/mouse. Each datum point represents the average number of CFU from organs of three or four mice and the standard deviation. (B) Infection of WT C57BL/6 mice with a moderately large dose (~2,000 CFU/mouse) of WT or lpqS::ΦMycoMarT7 (MHD131) M. tuberculosis. Because of the slow movement and labored breathing of the mice infected with the lpqS mutant, they were sacrificed at day 26. N.D., not determined.