FIG 3 .
The transposon insertion in MHD131 increased the expression of mmcO. (A) Map of the lpqS locus in strain MHD131. The ΦMycoMarT7 transposon was inserted at nucleotide (nt) 130 of lpqS. lpqS is 393 nt long. (B) Immunoblot analysis showed that MmcO increased upon 4 h of Cu treatment or in a ricR mutant (left). Data are representative of three biological replicates. In another experiment, robust induction of MmcO was observed after 24 h of treatment with 50 µM CuSO4 (right). (C) MmcO levels were higher in the lpqS mutant than in WT M. tuberculosis. Immunoblotting for dihydrolipoamide acetyltransferase (DlaT) was used as a loading control for all experiments.