FIG 4 .

Reovirus inclusions codistribute with ER and ERGIC elements. (A to F) HeLa cells were infected with T3-T1M1 for 12 h (A to C) or 24 h (D to F). Cells were fixed; permeabilized; stained for σNS (green), PDI (red), or nuclei (blue); and visualized by confocal microscopy. Arrows indicate viral inclusions associated with RER elements on the periphery. (G to L) HeLa cells (G to I) and MDCK cells (J to L) were infected with T3-T1M1 for 12 h; fixed; permeabilized; stained for dsRNA (red), ERGIC-53 (magenta), σNS (green), or nuclei (blue); and visualized by confocal microscopy. Arrows indicate viral inclusions that contain dsRNA, the ERGIC, and σNS. Scale bars: 10 µm.