FIG 5 .

3D model of reovirus inclusions in HeLa cells. HeLa cells were infected with T3-T1M1 and fixed at 12 hpi. The inclusion was visualized by TEM of serial sections, 3D reconstruction, and image processing. (A) Mitochondria (red) surround a network of membranes (yellow). RER (brown) and nuclear envelope (blue) are adjacent to the inclusion. Filled viral particles (black), empty viral particles (white), and microtubules (green) are integrated into the inclusion membrane network. (B) Rotation of the same reconstruction showing contacts between the inclusion membranes and mitochondria (arrows). The volume has been superimposed onto the 2D image of one of the sections in the series.