FIG 5 .

Substitutions in MERS-CoV spike proteins. All available KSA MERS-CoV spike ORFs were translated, the proteins aligned, and amino acid differences from the reconstructed ancestral clade B protein determined; changes observed in more than one genome are marked by vertical colored bars, with the new amino acid residue coded as shown at the bottom. Gray bars indicate a gap in sequence coverage. Functional domains of the spike (S) protein are marked and include the N-terminal domain, the receptor binding domain, the fusion domain (Fusion), heptad repeats 1 and 2 (HR1 and HR2) (20, 42), the transmembrane (TM) domain, and the cytoplasmic (Endo) domain (43).