Figure 1.

Fluorescence imaging of zebrafish larvae. (a) Cartoon of the experimental setup showing how the different modules are assembled onto the microscope for the simultaneous use of confocal Raman spectroscopy and fluorescence imaging. (b) Schematic representation of the optical path. (c) Fluorescence image of calcium-containing tissues, and fluids stained with calcein blue and excited at 400 nm (top). Endothelial cells of transgenic tg(fli1:EGFP)y1 zebrafish excited at 490 nm (bottom).