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Correlative fluorescence-Raman imaging of zebrafish fin bone maturation. (a) Low-resolution (10×) fluorescence image of zebrafish stained with calcein green, with high-resolution (60×) details (right inset in panel b) of a representative fin ray region where Raman spectra (b) of progressively mineralized bone tissue were acquired (numbered 1–4). (Left inset in panel b) Integral of the orientation independent mineral band (v2) where a clear drop of the mineral content can be observed.
