Temperature dependence of quadrupole splittings. The quadrupole coupling data were obtained from the C4′ (a), C8′ (b), C12′ (c), and C16′ (d) positions of PSPC (red) and SSM (blue) in the presence and absence of 50 mol % Chol. The temperature dependence of Chol-containing membranes can be linearly approximated, as presented in red and blue lines with approximate equations. To see this figure in color, go online.