Table. Immunofluorescence antibody titers to Anaplasma phagoctophilum in serum from 4 patients with human granulocytic anaplasmosis and reactive rP44 protein species, Japan, 2010–2011*.
| Patient no. | Time after illness onset, d | Antigen |
||||
|---|---|---|---|---|---|---|
|
A. phagocytophilum propagated in THP-1 cells (rP44 species) |
A. phagocytophilum propagated in HL60 cells (rP44 species) |
|||||
| IgM | IgG | IgM | IgG | |||
| 1 | 1 | 80 (r60) | <20 | 80 (r18ES) | <20 | |
| 15 | 160 (r60) | <20 | 160 (r18ES) | <20 | ||
|
|
30 |
320 (r60) |
20 (r60) |
|
320 (r18ES) |
<20 |
| 2 |
13 |
40 |
40 (r47E) |
|
<20 |
20 |
| 3 | 3 | 40 | 80 (r60) | <20 | 20 (r18ES) | |
| 7 | 40 | 80 (r60) | <20 | 20 (r18ES) | ||
| |
24 |
80 (r60) |
160 (r60) |
|
<20 |
40 (r18ES) |
| 4 | 4 | 160 (r47E) | 40 | <20 | <20 | |
| 15 | 160 (r47E) | 80 | <20 | <20 | ||
*Three recombinant P44 (rP44) protein species (r18ES, r47E, r60) were prepared and either one bound to antibodies in each serum sample from 4 patients in Western blot analyses (online Technical Appendix Figure 4, wwwnc.cdc.gov/EID/article/20/3/13-1337-Techapp1.pdf). r18ES represents rP44–18ES immunoreactive outer membrane protein that is known to predominate in A. phagocytophilum cultured in HL60 cells (6). r47E and r60 show rP44–47E and rP44–60 proteins, respectively, that are dominantly transcribed in A. phagocytophilum propagated in THP-1 cells (2).