Table 2. Quantitative reverse transcription PCR detection of Hendra virus N gene in samples collected daily from control horses*.
| Horse no., sample | log10 relative copy number of Hendra virus N RNA, dpc |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
| 1† | ||||||||||
| Blood | − | − | − | − | − | −0.2 | 1.4 | 1.7 | ||
| Urine | − | − | − | − | − | − | −0.8 | 1.1 | ||
| Feces | − | − | − | − | − | − | − | −0.7 | ||
| Nasal swab | − | − | −0.3 | −0.2 | 1.4 | 1.6 | 1.4 | 1.7 | ||
| Oral swab |
− |
− |
− |
− |
− |
−0.1 |
1.3 |
1.3 |
|
|
| 2‡ | ||||||||||
| Blood | − | − | − | − | 0.5 | 2.6 | 3.0 | |||
| Urine | − | − | − | − | − | 0.3 | 1.7 | |||
| Feces | − | − | − | − | − | −0.05 | 2.0 | |||
| Nasal swab | − | − | 1.2 | 2.0 | 1.6 | 3.5 | 2.5 | |||
| Oral swab |
− |
− |
− |
− |
− |
0.4 |
1.5 |
|
|
|
| 3‡ | ||||||||||
| Blood | − | − | − | − | − | − | 1.5 | 2.8 | 2.9 | 3.4 |
| Urine | − | − | − | − | − | − | − | 1.8 | 1.7 | 3.0 |
| Feces | − | − | − | − | − | − | − | 1.5 | 1.7 | 2.1 |
| Nasal swab | − | − | 1.7 | 2.5 | 1.2 | 2.4 | 3.0 | 3.8 | 3.7 | 2.0 |
| Oral swab |
− |
− |
− |
− |
− |
− |
− |
1.9 |
1.9 |
2.3 |
| 4‡ | ||||||||||
| Blood | − | − | − | − | − | 0.1 | 1.9 | 2.5 | 3.0 | |
| Urine | − | − | − | − | − | − | 0.07 | 0.5 | 2.1 | |
| Feces | − | − | − | − | − | − | 1.3 | 2.4 | 2.1 | |
| Nasal swab | − | − | 0.3 | − | − | − | − | 1.6 | 2.5 | |
| Oral swab | − | − | − | − | − | − | 0.2 | 1.2 | 1.6 | |
*Duplicate samples were obtained and tested by reverse transcription PCR. Cycle threshold values were converted to relative copy numbers by using a standard curve of a sample with a known copy number. dpc, days after challenge. − indicates a negative result; blank space indicates no sample was tested. †N gene data for horse 1 was obtained from the current study. ‡N gene data for horses 2–4 are unpublished data from a previous study (6).