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. Author manuscript; available in PMC: 2015 Jan 31.
Published in final edited form as: J Immunol Methods. 2013 Dec 2;403(0):17–25. doi: 10.1016/j.jim.2013.11.013

Table 2.

Inhibition of BTK-94C binding to brominated albumin by modified tyrosine analogs.

Chemical1 Structure Inhibition (IC50, µM) of BTK-
94C binding to brominated BSA2
Tyr graphic file with name nihms544862t1.jpg N.D.3
3-NO2-Tyr graphic file with name nihms544862t2.jpg N.D.
3-OH-HBA graphic file with name nihms544862t3.jpg N.D.
3-Br-HBA graphic file with name nihms544862t4.jpg 2 (0.3–6.3)
3,5-Di-Br-HBA graphic file with name nihms544862t5.jpg 0.7 (0.2–1.5)
3-Cl-Tyr graphic file with name nihms544862t6.jpg 107.8 (70.6–145.3)
3,5-Di-Cl-HBA graphic file with name nihms544862t7.jpg 113.6 (53.4–550.8)
1

Abbreviations: Tyr: L-Tyrosine; 3-NO2-Tyr, 3-nitrotyrosine; 3-OH-HBA, 3,4-dihydroxybenic acid; 3-Br-HBA, 3-bromo-4-hydroxybenzoic acid; 3,5-DiBr-HBA, 3,5-dibromo-4-hydroxybenzalehyde; 3-Cl-Tyr, 3-chlorotyrosine; and DiCl-HBA, 3,5-dichloro-4-hydroxybenoic acid.

2

The IC50 value is the median of triplicate analyses, each of which was fit to 3-parameter logistic binding curves. The 90% confidential intervals are shown are shown in parentheses.

3

N.D., inhibition was not detectable at any concentration examined (maximal concentration 1 mM).