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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Anal Biochem. 2013 Dec 10;449:17–25. doi: 10.1016/j.ab.2013.12.005

Figure 1.

Figure 1

2D-heteronuclear correlation spectra provide the foundation for all biomolecular NMR studies, but traditional 1H-detected methods do not work as well for IDPs as newer 13C-detected methods. (A) The 1H,15N-HSQC of Pdx1-C (B) The 15N,13C-CON of Pdx1-C. Of special value is the presence of peaks corresponding to proline residues, seen downfield shifted in the nitrogen dimension of the 15N,13C-CON. Note that the amino acid sequence of Pdx1-C is provided at the top of the figure, with the N-terminal GPG cloning artifact shown in the thinner font. A larger version of panel (B) is provided in Fig. S1, including complete annotation of chemical shift assignments.