Table 2.
Analyzed murine genes and their specific real-time QPCR conditions
| Mouse genes | Accession number (NCBI) | Oligonucleotides (sense/antisense) | Amplicon length (bp) |
QPCR conditions
a
|
|
|---|---|---|---|---|---|
| Ann. T (°C) | Acq. T (°C) | ||||
|
Cyp11a1 |
NM_019779 |
ATCCGGGCTTCTTTCCCAATC/GGATGGGGTTCTCAGGCATC |
249 |
64 |
86 |
|
Hsd3b1 |
NM_008293 |
TGCCAGGGCATCTCTGTTGTC/TCTGTTCCTCGTGGCCATTCA |
220 |
64 |
84 |
|
Cyp21a1 |
NM_009995 |
TCACGACTGTGTCCAGGACTTG/TTCGTCTTTGCCATCCCTTTG |
250 |
67 |
84 |
|
Akr1C18 |
NM_1340662.2 |
GCACCATAGGCAACCAGAAC/TCTCATTCATTTCCCAGTGTCTC |
312 |
54 |
78 |
|
Cyp11b1 |
NM_001033229 |
CTGGGACAGTCCTCAATGTGA/ATCCGCACATCCTCTTTCTCTT |
244 |
62 |
87 |
|
Hsd11b1 |
NM_008288 |
GGCCAGCAAAGGGATTGGAAG/TTTTCCCAGCCAAGGAGGAGA |
401 |
66 |
85 |
|
Hsd11b2 |
NM_008289 |
TGGCTGACGTGGGACTGTCT/TTGGAGCAGCCAGGCTTGATA |
277 |
63 |
87 |
|
Gapdh |
NM_008084 |
GTCGGTGTGAACGGATTTG/AAGATGGTGATGGGCTTCC |
215 |
61 |
84 |
| Hmbs | NM_013551 | GGAATGCATGTATGCTGTGGG/CAGGTACAGTTGCCCATCTT | 208 | 59 | 85 |
aAnn. T, annealing temperature; Acq. T, acquisition (fluorescence intensity reading) temperature.