Figure 4.

Transient induction of inhibitory Smad7 in the lungs. Representative images of Smad7 levels in lung tissue at 9 h (a and b) and 24 h (c and d) following sham operation (a and c) and acute pancreatitis induction (b and d). Results from one representative of eight samples per group are shown. Original magnification, ×20. Western blot analysis of Smad7 protein levels in lung extracts of sham-operated and ligated animals at two time points (e). Two of five similar analyses per group are shown. Graph displays densitometry measurement of fold difference in Smad7 levels compared to sham control and represents mean ± SEM of five samples per group (e). *P < 0.05.