Detection of CTG repeat instability in blood from a 27 year-old DM1 patient carrying more than 170 CTG repeat.

SP-PCR was performed as described by Gomes-Pereira et al, 2004. An average of 250, 50 and 10 genome equivalents (g.e.) were amplified in each replicate reaction, as indicated above the lanes. The membrane was hybridized with a non-radioactive CAG oligonucleotide probe. The medical RX autoradiography film was exposed for 5 minutes at room temperature.